Modifying Enzymes
Worthington Biochemical Corporation PHOSPHODIESTERASE II 10UN
Prepared from the 1 mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe, Biochem. Prep, 8, (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961). Lyophilized in vials. 9068-54-6
Cusabio Technology LLC Fish Catalase CAT ELISA kit
The Fish Catalase (CAT) ELISA Kit is designed for the quantitative detection of catalase (CAT) in fish serum and plasma samples using a competitive ELISA method This kit detects CAT within a broad dynamic range of 125 mU/mL to 2000 mU/mL with a sensitivity of 62 5 mU/mL ensuring precise measurement of enzyme activity The assay requires a short incubation period of 1 to 5 hours and utilizes a minimal sample volume of 50-100 L per test Optical density readings are performed at 450 nm providing reliable and reproducible results for research applications Ideal for studying oxidative stress biomarkers this kit offers a robust and efficient tool for quantifying CAT levels in aquatic species
Cayman Chemical CISD1 CytosolIc DomaInhumn
Source: Recombinant human N-terminal His-tagged CISD1 expressed in E. coli • Amino acids: 32-108 • MW: 11.3 kDa
Cayman Chemical N-Oleoyl-DPPEamonIum salt 5mg
An N-acyl-phosphatidylethanolamine; a biosynthetic precursor to OEA
Apexbio Technology LLC EZ Cap ER-EGFP Probe mRNA (m1Ψ) 5x1mg (1mg/mL)
ER-EGFP mRNA encodes a fusion protein comprising an endoplasmic reticulum (ER) targeting signal sequence and enhanced green fluorescent protein (EGFP) EGFP is a widely used fluorescent marker protein for biological imaging By fusing EGFP to the ER signal sequence dynamic changes in the ER can be observed in real-time in live cells making it particularly useful for studying the structure function and interactions of the ER with other cellular organelles ER-EGFP s efficient fluorescent properties and specific localization to the ER position it as an ideal tool for studying ER-related processes The EZ Cap ER-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL specifically designed for targeted expression of a green fluorescent protein label at the ER This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
ABclonal Technology T4 RNA Ligase 2, truncated
T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5 end of DNA or RNA to the 3 end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2 truncated is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5 end of the substrate, and as a result it cannot ligate the phosphorylated 5? end of RNA or DNA to the 3? end of RNA. This enzyme, also known as Rnl2 (1-249) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.
Cayman Chemical N-Oleoyl-L-phenylalnIn 1mg
An N-acyl amide; increases the OCR of mitochondria isolated from mouse brown adipose tissue in a Seahorse assay and decreases oligomycin-induced increases in the mitochondrial membrane potential in C2C12 cells, indicating mitochondrial uncoupling activity at 50 µM; reduces food intake and body weight of diet-induced obese mice at 30 mg/kg for 8 days
INTACT GENOMICS INC Exonuclease III 10000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios (3-5).Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
Enzo Life Sciences Ubiquitin activating enzyme E1 (human), (recombinant) (His-tag) (50 µg)
Essential ubiquitinylation pathway enzyme. MW: 118 kDa. Purity: ≥90% (SDS-PAGE). Formulation: In 50mM TRIS-hydrochloric acid, containing 1.0mM dithiothreitol. Source: Produced in E. coli BL21 (λDE3) expression system and containing a C-terminal His6-tag. Full length human ubiquitin-activating enzyme E1. UniProt: P22314. Handling: Avoid freeze/thaw cycles. Long Term Storage: -80°C
Cambridge Isotope Laboratories LYSO-PC 26:0 (HEXACOSANOYL-1,2,3,4,5,6-13C6, 99%), 5 MG
LYSO-PC 26:0 (HEXACOSANOYL-1,2,3,4,5,6-13C6, 99%), 5 MG
Carnabio Usa Inc WNK3/10UG/CATALYTIC
WNK3, Catalytic domain, Wild type, Amino Acid 1-434, NP_065973.2, Expressed in Insect (sf21); 10microg
ABclonal Technology T7 DNA Ligase
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It can catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl termini in double-stranded DNA. T7 DNA ligase can catalyze ligation of cohesive ends and repair nicks, but not blunt end ligation. This makes a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined .
Cayman Chemical N-Oleoyl ValIn 50mg
An endogenous N-acyl amine; antagonist at TRPV3; increases after acute lung injury in mouse lung tissue
ABclonal Technology T4 RNA Ligase 2, truncated
T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5 end of DNA or RNA to the 3 end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2 truncated is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5 end of the substrate, and as a result it cannot ligate the phosphorylated 5? end of RNA or DNA to the 3? end of RNA. This enzyme, also known as Rnl2 (1-250) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.
Cayman Chemical EthylmaLNyl Coenzyme AsodI 5mg
An intermediate in the ethylmalonyl-CoA pathway in bacteria